vital stain: [ stān ] 1. a substance used to impart color to tissues or cells, to facilitate microscopic study and identification. Hematoxylin reacts like a basic dye with a purplish blue colour. Internationally, several ophthalmic vital dyes are now available, although only one formulation is currently approved for ophthalmic use by the US Food and Drug Administration (FDA). Doerte Luensmann, Amir Moezzi, Rachael Claire Peterson, Craig Woods, Desmond Fonn, Corneal Staining and Cell Shedding during the Development of Solution-Induced Corneal Staining, Optometry and Vision Science, 10.1097/OPX.0b013e318255da58, 89, 6, (868-874), (2012). In supravital staining, living cells have been removed from an organism, whereas intravital staining is done by injecting or otherwise introducing the stain into the body. Virtually all stains, even the so-called vital stains, are eventually toxic to the organisms. The H&E stain uses two dyes, hematoxylin and eosin. It stains acidic, or basophilic, structure including the cell nucleous (which contains DNA and nucleoprotein), and organelles that contain RNA such as ribosomes and the rough endoplasmic reticulum. A vital stain in a casual usage may mean a stain that can be applied on living cells without killing them. Vital stains have been useful for diagnostic and surgical techniques in a variety of medical specialties. Evans blue (EB) dye has owned a long history as a biological dye and diagnostic agent since its first staining application by Herbert McLean Evans in 1914. These stainings, first established on planctonic bacteria, may have serious shortcomings when applied to multispecies biofilms. For the amateur microscopist, the use of vital stains can provide additional information about the cytological details of protozoa. Staining is a term that describes the epithelial disruption and other pathophysiologic changes observed when using topical dyes––also referred to as vital stains. differential stain one that facilitates differentiation of various elements in a specimen. “Viability staining” or “vital staining techniques” are used to distinguish live from dead bacteria. The technique is based on the principle that dead cells (i.e. 2. an area of discoloration of the skin. 1 Vital stains allow visualization of tissues in their living state. Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA.Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. After treatment for 60 min at 30 degrees C, FM 4-64 stained the vacuole membrane (ring staining pattern). It is an optimized staining technique for assessment of sperm vitality, which is one of the basic tools used in semen analysis. Vital staining is the clinical ly visible aid that can be used routinely to assist the site of biopsy, follow up of premalignant lesions and marginal demarcation of the malignant lesions enables an those with damaged plasma membrane) will take up the eosin and stain red. acid-fast stain a staining procedure for demonstrating acid-fast microorganisms. Vital dyes should also offer repeatable, avid staining, yet be easily removable. We have used a lipophilic styryl dye, N-(3-triethylammoniumpropyl)-4- (p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM 4-64), as a vital stain to follow bulk membrane-internalization and transport to the vacuole in yeast. This combination is used as the dyes stain different tissue elements. There is confusion over the definition of the term “viability state(s)” of microorganisms. 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